Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy

Moers, M.H.P. and Ruiter, A.G.T. and Jalocha, A. and Hulst van, N.F. (1995) Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy. Ultramicroscopy, 61 (1-4). pp. 279-283. ISSN 0304-3991

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Abstract:	Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single chromosome with a resolution superior to traditional fluorescence microscopy. Several nucleic acid probes have been used. The hybridization signals are well resolved in the near- field fluorescence images, and the exact location of the probes can be correlated to the topography as it is afforded by the shear-force feedback.
Item Type:	Article
Copyright:	© 1995 Elsevier Science
Research Group:	Optical Sciences (OS)
Link to this item:	http://purl.utwente.nl/publications/24565
Official URL:	http://dx.doi.org/10.1016/0304-3991(96)85133-3
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Metis ID: 129366