Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy

Moers, M.H.P. and Kalle, W.H.J. and Ruiter, A.G.T. and Wiegant, J.C.A.G. and Raap, A.K. and Greve, J. and Grooth de, B.G. and Hulst van, N.F. (1996) Fluorescence in situ hybridization on human metaphase chromosomes detected by near-field scanning optical microscopy. Journal of Microscopy, 182 (pt. 1). pp. 40-45. ISSN 0022-2720

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Abstract:	Fluorescence in situ hybridization on human metaphase chromosomes is detected by near-field scanning optical microscopy. This combination of cytochemical and scanning probe techniques enables the localization and identification of several fluorescently labelled genomic DNA fragments on a single chromosome with an unprecedented resolution. Three nucleic acid probes are used: pUC1.77, p1-79 and the plasmid probe alpha-spectrin. The hybridization signals are very well resolved in the near-field fluorescence images, while the exact location of the probes can be correlated accurately with the chromosome topography as afforded by the shear force image.
Item Type:	Article
Copyright:	© 1996 The Royal Microscopical Society
Research Group:	Optical Sciences (OS)
Link to this item:	http://purl.utwente.nl/publications/24562
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