Dielectric and fluorescent samples imaged by scanning near-field optical microscopy in reflection

Jalocha, A. and Hulst van, N.F. (1995) Dielectric and fluorescent samples imaged by scanning near-field optical microscopy in reflection. Optics Communications, 119 (1-2). pp. 17-22. ISSN 0030-4018

Preview

PDF
1181Kb

Abstract:	Dielectric fluorescent samples are imaged by scanning near- field optical microscopy in reflection. A non-metallized tapered fibre tip is used both as an emitter and a detector. Shear force feedback controls the distance between the tip and the sample and gives simultaneously a topographic image of the surface. A direct correlation with the optical image is obtained. We demonstrate that this reflection setup is suitable for dielectric samples. Images in fluorescence have been obtained on Langmuir-Blodgett films. For fluorescence detection the optical contrast is unambiguous, and consequently we estimate the detecting size of the probe at about 200 nm. Operation in reflection opens new possibilities since it allows the study of metallic, thick, opaque or dielectric sample and can be easily combined with a transmission setup. In this way, biological samples can be imaged optically simultaneously in transmission, reflection and with a shear force control.
Item Type:	Article
Copyright:	© 1995 Elsevier Science
Research Group:	Optical Sciences (OS)
Link to this item:	http://purl.utwente.nl/publications/24553
Official URL:	http://dx.doi.org/10.1016/0030-4018(95)00276-E
Export this item as:	BibTeX EndNote HTML Citation Reference Manager

Show download statistics for this publication

Repository Staff Only: item control page

Metis ID: 129354