Near-Field Scanning Optical Microscopy of Single Fluorescent Dendritic Molecules

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Veerman, Joost A. and Levi, Stefano A. and Veggel van, Frank C.J.M. and Reinhoudt, David N. and Hulst van, Niek F. (1999) Near-Field Scanning Optical Microscopy of Single Fluorescent Dendritic Molecules. Journal of Physical Chemistry A, 103 (51). pp. 11264-11270. ISSN 1089-5639

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Abstract:Individual dendritic molecules adsorbed o­n glass containing a single fluorescent rhodamine B core have been observed with near-field scanning optical microscopy (NSOM); height and fluorescence images were obtained simultaneously. The dendritic assemblies can be discriminated from free fluorescent cores o­n the basis of accurate simultaneous localization of both the fluorescent core and the surrounding dendritic shell. There are no significant differences between the photophysical properties of the free and dendritic fluorophores. The full three- dimensional orientation of each individual fluorescent core can be resolved and millisecond time resolution accuracy has been achieved. Most dendritic structures exhibited rotational motion of the fluorescent core o­n a millisecond-second time scale, revealing intramolecular conformational dynamics
Item Type:Article
Copyright:© 1999 American Chemical Society
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Link to this item:http://purl.utwente.nl/publications/23823
Official URL:http://dx.doi.org/10.1021/jp992635u
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Metis ID: 128622