Chromatin Structure in Bands and Interbands of Polytene Chromosomes Imaged by Atomic Force Microscopy

Grauw de, C.J. and Avogadro, A. and Heuvel van den, D.J. and Werf van der, K.O. and Otto, C. and Kraan, Y. and Hulst van, N.F. and Greve, J. (1998) Chromatin Structure in Bands and Interbands of Polytene Chromosomes Imaged by Atomic Force Microscopy. Journal of Structural Biology, 121 (1). pp. 2-8. ISSN 1047-8477

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Abstract:	Polytene chromosomes from Drosophila melanogaster, observed from squash preparations, and chromosomes from Chironomus thummi thummi, investigated under physiological conditions, are imaged using an Atomic Force Microscope. Various chromatin fiber structures can be observed with high detail in fixed chromosomes and correspond to structures which are also observed in chromosomes of diploid cells. Unfixed chromosomes can be imaged in buffer and show less fiber-like details because of the inherent soft nature of the chromatin material.
Item Type:	Article
Copyright:	© 1998 Academic Press
Faculty:	Science and Technology (TNW)
Research Group:	Medical Cell BioPhysics (MCBP)
Link to this item:	http://purl.utwente.nl/publications/23635
Official URL:	http://dx.doi.org/10.1006/jsbi.1997.3929
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Metis ID: 128434