Adhesion and neurite development of cortical neurons on micropatterns of polyethylenimine and fluorcarbon

Ruardij, T.G. and Goedbloed, M.H. and Rutten, W.L.C. (2000) Adhesion and neurite development of cortical neurons on micropatterns of polyethylenimine and fluorcarbon. In: 1st Annual International Conference On Microtechnologies in Medicine and Biology, Lyon, France.

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Abstract:	This study aims on the preparation of isolated islands of cortical neurons on modified glass surfaces. Isolated islands of cortical neurons were obtained with a combination of neuron-adhesive polyethylenimine (PEI) and neuron-repellent plasma-deposited fluorocarbon (FC). Neurite development and fasciculation of interconnecting neurites between PEI-coated areas was studied. The patterns consisted of PEI-coated wells (diameter 150 ¿m) which were initially etched in a Fluorocarbon (FC) layer. The separation distance between the PEI-coated wells was varied from 10 up to 90 ¿m. This paper shows that the chemical patterns, prepared with a combination of polyethylenimine (PEI) and plasma deposited Fluorocarbon (FC), results in highly compliant patterns of adhering cortical neurons. Furthermore, it was shown that the occurrence of connecting neurite fascicles between neurons on PEI-coated wells is inversely proportional to the separation distance between the wells. Interconnecting fascicles were especially present on the pattern with a minimal separation distance (10 ¿m) between the PEI-coated wells. In contrast, interconnecting neurite fascicles were not observed on patterns with a minimal separation distance of 80 ¿m between the wells
Item Type:	Conference or Workshop Item
Copyright:	©2000 IEEE
Faculty:	Electrical Engineering, Mathematics and Computer Science (EEMCS)
Research Group:	Biomedical Signals and Systems (BSS)
Link to this item:	http://purl.utwente.nl/publications/16433
Official URL:	http://dx.doi.org/10.1109/MMB.2000.893863
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