Development of an ISFET based heparin sensor using the ion-step measuring method

Kerkhof van, J.C. and Bergveld, P. and Schasfoort, R.B.M. (1993) Development of an ISFET based heparin sensor using the ion-step measuring method. Biosensors and Bioelectronics, 8 (9-10). pp. 463-472. ISSN 0956-5663

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Abstract:	The ion-step measuring method was used to determine heparin concentrations in PBS and blood plasma. Heparin is a sulphated polysaccharide which is clinically used as a drug to prevent the clotting of blood. The measuring method is based on detection of changes in charge density in a porous membrane which is deposited on the gate of an ISFET. Protamine was used as affinity ligand in the membrane. In PBS a linear relation was found between the heparin concentration and the ISFET response. The incubation time was reduced from 18 h to 15 min by increasing the porosity of the membrane. The results of the measurements in blood plasma show a significant nonspecific binding of plasma components in the membrane. Suggestions are given to prevent this nonspecific adsorption. The results described in this paper show a detection limit for the ion-step measuring method of at least 5 ± 10-11 Mol/l which is promising for future practical applications.
Item Type:	Article
Copyright:	© 1993 Elsevier Science
Faculty:	Science and Technology (TNW)
Research Group:	Medical Cell BioPhysics (MCBP)
Link to this item:	http://purl.utwente.nl/publications/15049
Official URL:	http://dx.doi.org/10.1016/0956-5663(93)80031-J
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