Adhesion and patterning of cortical neurons on polyethylenimine and fluorocarbon-coated surfaces

Ruardij, T.G. and Goedbloed, M.H. and Rutten, W.L.C. (2000) Adhesion and patterning of cortical neurons on polyethylenimine and fluorocarbon-coated surfaces. IEEE transactions on biomedical engineering, 47 (12). pp. 1593-1599. ISSN 00189294

Preview

PDF
175Kb

Abstract:	Adhesion and patterning of cortical neurons was investigated on isolated islands of neuron-adhesive polyethylenimine (PEI) surrounded by a neuron-repellent fluorocarbon (FC) layer. In addition, the development of fasciculated neurites between the PEI-coated areas was studied over a time period of 15 days. The patterns consisted of PEI-coated wells (diameter 150 /spl mu/m, depth 0.5 /spl mu/m) which were etched in a coating of fluorocarbon (FC) on top of polyimide (PI) coated glass. The separation distance between the PEI-coated wells were varied between 10 and 90 /spl mu/m. This paper shows that chemical patterns of PEI and FC result in highly compliant patterns of adhering cortical neurons after 1 day in vitro. Interconnecting neurite fascicles between PEI-coated wells were especially present on patterns with a separation distance of 10 /spl mu/m after 8 days in vitro. A significant lower number of interconnecting neurite fascicles was observed on 20 /spl mu/m separated patterns. Effective isolation of neurons into PEI-coated wells was achieved on patterns with a separation distance of 80 /spl mu/m as no interconnecting neurite fascicles were observed.
Item Type:	Article
Copyright:	©2000 IEEE
Faculty:	Electrical Engineering, Mathematics and Computer Science (EEMCS)
Research Group:	Biomedical Signals and Systems (BSS)
Link to this item:	http://purl.utwente.nl/publications/14736
Official URL:	http://dx.doi.org/10.1109/10.887940
Export this item as:	BibTeX EndNote HTML Citation Reference Manager

Show download statistics for this publication

Repository Staff Only: item control page

Metis ID: 111762