Crown ether activation of cross-linked subtilisin Carlsberg crystals in organic solvents

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Unen van, Dirk-Jan and Sakodinskaya, Inna K. and Engbersen, Johan F.J. and Reinhoudt, David N. (1998) Crown ether activation of cross-linked subtilisin Carlsberg crystals in organic solvents. Journal of the Chemical Society. Perkin transactions I, 1998 (20). pp. 3341-3343. ISSN 0300-922X

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Abstract:The activity of cross-linked subtilisin Carlsberg crystals in the catalysis of peptide bond formation can be significantly enhanced by pretreatment of the enzyme crystals with crown ethers. Soaking of the enzyme crystals in a solution of crown ether in acetonitrile followed by evaporation of the solvent results in an up to 13 times enhanced enzymatic activity. The effects of crown ether treatment under various conditions gives support for the hypothesis that removal of bound water molecules from the active site during the drying process is the origin of the observed enzyme activation.
Item Type:Article
Copyright:© 1998 Royal Society of Chemistry
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Science and Technology (TNW)
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Link to this item:http://purl.utwente.nl/publications/11062
Official URL:http://dx.doi.org/10.1039/a806294c
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Metis ID: 105975